Journal: PLoS ONE

Article Title: Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model

doi: 10.1371/journal.pone.0173261

Figure Lengend Snippet: Creatine/weight ratio of human control muscles and exclusion criteria for muscle specimen. Unit was expressed in μmol of creatine/g of wet tissue weight. The cutoff value was defined as mean creatine/ratio minus 1SD (Table 1A). Total number of control and GNEM biopsy specimens available and number of specimens that met the cutoff criteria were listed in the Tables 1B and 1C.

Article Snippet: Control human deltoid muscle samples from autopsy donors without a history of muscle disease (2 males and 3 females, range 60–69 years old) were obtained from BioreclamationIVT.

Techniques: Control, Muscles

Journal: PLoS ONE

Article Title: Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model

doi: 10.1371/journal.pone.0173261

Figure Lengend Snippet: Summary of free and total SA results in serum and muscle for human and mouse data. Sialic acid values were shown as mean normalized values with SE. Change of SA in GNEM patients or Gne M743T/M743T mice from corresponding controls were shown as percentage change. Statistical not significant change was represented as p = NS.

Article Snippet: Control human deltoid muscle samples from autopsy donors without a history of muscle disease (2 males and 3 females, range 60–69 years old) were obtained from BioreclamationIVT.

Techniques:

Journal: PLoS ONE

Article Title: Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model

doi: 10.1371/journal.pone.0173261

Figure Lengend Snippet: Quads = quadriceps, GS = gastrocnemius. A) All available samples. B) Samples remained after excluding specimens with low creatine/weight ratio as defined in .

Article Snippet: Control human quadriceps muscle samples (vastus lateralis) from autopsy donors without a history of muscle disease (8 males and 4 females, range 34–66 years old) were obtained from BioreclamationIVT (Westbury, NY, http://www.bioreclamationivt.com ) and Integrated Laboratory Services Biotech (Charlestown, MD, http://www.ils-inc.com ).

Techniques:

Journal: PLoS ONE

Article Title: Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model

doi: 10.1371/journal.pone.0173261

Figure Lengend Snippet: A) Free SA data after excluding specimens with low creatine/weight ratio as defined in . * -indicates a statistical significant difference with a p<0.001 for quadriceps (quads), p = 0.025 for gastrocnemius (GS), p = 0.002 for deltoid. B) Total SA data after excluding specimens with low creatine/weight ratio as defined in . # -indicates a statistical significant difference with a p = 0.02 for quads, p = 0.008 for deltoid. NS denotes not statistically significant. Free and total SA values were corrected for creatine (μg SA/μmol creatine).

Article Snippet: Control human quadriceps muscle samples (vastus lateralis) from autopsy donors without a history of muscle disease (8 males and 4 females, range 34–66 years old) were obtained from BioreclamationIVT (Westbury, NY, http://www.bioreclamationivt.com ) and Integrated Laboratory Services Biotech (Charlestown, MD, http://www.ils-inc.com ).

Techniques:

Journal: PLoS ONE

Article Title: Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model

doi: 10.1371/journal.pone.0173261

Figure Lengend Snippet: A) Free SA data in both littermate controls and Gne M743T/M743T mice. * -indicates a statistical significant difference with a p = 0.006 for quadriceps (quads), p = 0.005 for gastrocnemius (GS), p = 0.006 for biceps. B) Total SA data in both littermate controls and Gne M743T/M743T mice. # -indicates a statistical significant difference with a p = 0.01 for quadriceps (quads). NS denotes not statistically significant. Free and total SA values were corrected for protein (μg SA/mg protein).

Article Snippet: Control human quadriceps muscle samples (vastus lateralis) from autopsy donors without a history of muscle disease (8 males and 4 females, range 34–66 years old) were obtained from BioreclamationIVT (Westbury, NY, http://www.bioreclamationivt.com ) and Integrated Laboratory Services Biotech (Charlestown, MD, http://www.ils-inc.com ).

Techniques: